CRL-2408™
Explore our dataNK-92® MI are natural killer cells that are cytotoxic to a wide range of malignant cells; it kills both K562 cells and Daudi cells in chromium release assays. This cell line was derived from peripheral blood mononuclear cells from a 50-year-old, White male with rapidly progressive non-Hodgkin's lymphoma. Use this cell line in your immunology and cancer research.
- Product category
-
Human cells
- Organism
-
hom*o sapiens, human
- Cell type
- natural killer cell; nk cell
- Morphology
- lymphoblast
- Tissue
- Peripheral blood
- Disease
-
Lymphoma; Malignant Non-Hodgkin's
- Applications
-
3D cell culture
Immunology
- Product format
- Frozen
- Storage conditions
-
Vapor phase of liquid nitrogen
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These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Documentation
Certificate of Analysis Download
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Certificate of Origin Download
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This product sheet is not available online. We only provide this product sheet to customers who have purchased this biosafety level 3 product. If you purchased this product, please contact Technical Service for this product sheet.
Safety Data Sheet Download
Open the Safety Data Sheet for this product to download.
If a requested product is not a hazardous chemical, or does not contain any hazardous chemicals, a SDS is not required and therefore will not be provided.
Please check the Product Sheet and Safety Data Sheet Landing pagefor more information.
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
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Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Fetal Bovine Serum (FBS)
30-2020
Dimethylsulfoxide (DMSO)
4-X
Related Products
NK-92®
CRL-2407
Detailed product information
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General
- Specific applications
-
The cell line is cytotoxic to a wide range of malignant cells; it kills both K562 cells and Daudi cells in chromium release assays.
Characteristics
- Growth properties
- Suspension, multicellular aggregates
- Derivation
-
NK-92® MI is an interleukin-2 (IL-2) independent Natural Killer Cell line derived from the NK-92® (ATCC CRL-2407) cell line by transfection. NK-92® is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. The parental cells were transfected with human IL-2 cDNA in the retroviral MFG-hIL-2 vector by particle-mediated gene transfer. The transfection is stable.
- Age
- 50 years
- Ethnicity
- White
- Gender
- Male
- Comments
-
NK-92® and this derivative cell line NK-92® MI have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR.
The parental IL-2 dependent cell line is available as ATCC CRL-2407 (NK-92® ). NK-92® MI was shown to contain, express, and synthesize the hIL-2.
A culture submitted to the ATCC in September of 1998 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
Handling information
- Unpacking and storage instructions
-
- Check all containers for leakage or breakage.
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
- The complete medium for this cell line is MyeloCult™ H5100 (StemCell Technologies cat # 05150)
- Temperature
-
37°C
- Atmosphere
-
95% Air, 5% CO2
- Handling procedure
- To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
- Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
- Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
- Withdraw the cells from the ampoule and transfer thawed cells to empty 15 mL centrifuge tube. Add 9-11 mL 4°C culture medium slowly to the cell suspension.
- Centrifuge the cell suspension at approximately 175 x g for 10 minutes at 4°C. Discard the supernatant.
- Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into anew culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
- Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
- Subculturing procedure
-
Cultures can be maintained by centrifuging cells and resuspending cell pellet in fresh medium at 2 - 3 x 105 viable cells/mL. Centrifugation and full replacement of culture medium may be performed for the first subcultures. Cultures can then be maintained by addition of fresh medium. These cells tend to grow in aggregates that may lose viability when they are dispersed. Accurate counts and viabilities may not be possible.
Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL or use a 1:3 spilt ratio.
- Reagents for cryopreservation
- CryoStor® CS10 (StemCell Technologies at # 07930)
Quality control specifications
- Mycoplasma contamination
- Not detected
- Virus testing
-
Epstein-Barr virus (EBV): Detected
- STR profiling
- Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9,12 D16S539: 11,12 D5S818: 12,13 D7S820: 10,11 TH01: 6,9.3 TPOX: 8 vWA: 16,18 D3S1358: 15 D21S11: 31.2,32 D18S51: 12,17 Penta_E: 12 Penta_D: 10,12 D8S1179: 12,15 FGA: 20,22 D19S433: 14,15 D2S1338: 19,20
History
- Deposited as
- human
- Depositors
- ImmunityBio, Inc.
- Year of origin
- 1998
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
For-profit Research Use License from ImmunityBio, Inc.
The NK-92 cell line was deposited at ATCC by ImmunityBio, Inc. and is available with the following restrictions:
- Not-for-profit academic institutions may use this cell line for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes, including but not limited to the testing or validation of any commercial product or instrument. Nor can the cells be distributed to third parties for any reason, including purposes of sale or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.
- For-profit commercial organizations must obtain a research use and/or a commercial license directly from ImmunityBio, Inc. For instructions on how to proceed, please contact ImmunityBio, Inc.’s licensing department via email at [emailprotected]."
If sending the license to us, email the license to [emailprotected] with a reference to both your account and sales order numbers. Once either the license or authorization is received, your order will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS
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